The impacts of faecal subsampling on microbial compositional profiling.

ABSTRACT:

Objective

Despite the move to at-home, small-volume collection kits to facilitate large population-based studies of faecal microbial compositional profiling, there remains limited reporting on potential impacts of faecal subsampling approaches on compositional profiles. This study aimed to compare the microbial composition from faecal subsamples (< 5 g) collected from the beginning and end of a single bowel movement in ten otherwise healthy adults (6 female, 4 male; age: 24-55 years). Microbial composition was determined by V3-V4 16s rRNA sequencing and compared between subsamples.

Results

There were no significant differences in OTU count (p = 0.32) or Shannon diversity index (p = 0.29) between the subsamples. Comparison of relative abundance for identified taxa revealed ve

SUBMITTER: Cox AJ 

PROVIDER: S-EPMC8842933 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature