Biosynthesis of Diverse Type II Polyketide Core Structures in Streptomyces coelicolor M1152.

ABSTRACT: Synthetic biology-based approaches have been employed to generate advanced natural product (NP) pathway intermediates to overcome obstacles in NP drug discovery and production. Type II polyketides (PK-IIs) comprise a major subclass of NPs that provide attractive structures for antimicrobial and anticancer drug development. Herein, we have assembled five biosynthetic pathways using a generalized operon design strategy in Streptomyces coelicolor M1152 to allow comparative analysis of metabolite production in an improved heterologous host. The work resulted in production of four distinct PK-II core structures, namely benzoisochromanequinone, angucycline, tetracenomycin, and pentangular compounds, which serve as precursors to diverse pharmaceutically important NPs. Our bottom-up design

SUBMITTER: Zhu X 

PROVIDER: S-EPMC8843011 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature