A platform for detecting cross-resistance in antibacterial drug discovery.

ABSTRACT:

Background

To address the growing antibiotic resistance problem, new antibacterial drugs must exert activity against pathogens resistant to agents already in use. With a view to providing a rapid means for deselecting antibacterial drug candidates that fail to meet this requirement, we report here the generation and application of a platform for detecting cross-resistance between established and novel antibacterial agents.

Methods

This first iteration of the cross-resistance platform (CRP) consists of 28 strains of defined resistance genotype, established in a uniform genetic background (the SH1000 strain of the clinically significant pathogen Staphylococcus aureus). Most CRP members were engineered through introduction of constitutively expressed resistance determinants on

SUBMITTER: Galarion LH 

PROVIDER: S-EPMC8843079 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature