Amino acid substitution within the VP7 protein of G2 rotavirus strains associated with failure to serotype.

ABSTRACT: Rotavirus strains collected in the United Kingdom during the 1995-1996 season and genotyped as G2 by reverse transcription-PCR failed to serotype in enzyme-linked immunosorbent assays using three different G2-specific monoclonal antibodies. The deduced amino acid sequences of the antigenic regions A (amino acids 87 to 101), B (amino acids 142 to 152), and C (amino acids 208 to 221) of VP7 revealed that a substitution at position 96 (Asp-->Asn) correlated with the change in ability to serotype these G2 strains.

SUBMITTER: Gomara MI 

PROVIDER: S-EPMC88438 | biostudies-literature | 2001 Oct

REPOSITORIES: biostudies-literature