Non-enzymatic formation of isoprene and 2-methyl-3-buten-2-ol (2-MBO) by manganese.

ABSTRACT: It has been suggested that isoprene synthesis by isoprene synthase (IspS) proceeds via a substrate-assisted mechanism. The authors observed a non-enzymatic isoprene formation by Mn²⁺, which represents the basis of IspS enzyme reaction. Because IspS and many other terpene synthases require Mn²⁺ metal ions as cofactor, this study characterized the formation reaction for the first time. Metal ions including Mn²⁺ non-enzymatically produced both isoprene and 2-methyl-3-buten-2-ol (2-MBO) from dimethylallyl pyrophosphate (DMADP). Isoprene formation was most enhanced by Fe²⁺ and, to a lesser extent, by Mn²⁺ or Cu²⁺. Ni²⁺, Co²⁺, Mg²⁺, and Ba²⁺ exhibited a low activity to generate both isop

SUBMITTER: Oku H 

PROVIDER: S-EPMC8844067 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature