Tick tock, tick tock: Mouse culture and tissue aging captured by an epigenetic clock.

ABSTRACT: Aging is associated with dramatic changes to DNA methylation (DNAm), although the causes and consequences of such alterations are unknown. Our ability to experimentally uncover mechanisms of epigenetic aging will be greatly enhanced by our ability to study and manipulate these changes using in vitro models. However, it remains unclear whether the changes elicited by cells in culture can serve as a model of what is observed in aging tissues in vivo. To test this, we serially passaged mouse embryonic fibroblasts (MEFs) and assessed changes in DNAm at each time point via reduced representation bisulfite sequencing. By developing a measure that tracked cellular aging in vitro, we tested whether it tracked physiological aging in various mouse tissues and whether anti-aging interventions modulat

SUBMITTER: Minteer C 

PROVIDER: S-EPMC8844113 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature