A protocol for full-rotation soft X-ray tomography of single cells.

ABSTRACT: The protocol describes step-by-step sample preparation, data acquisition, and segmentation of cellular organelles with soft X-ray tomography. It is designed for microscopes built to perform full-rotation data acquisition on specimens in cylindrical sample holders, such as the XM-2 microscope at the Advanced Light Source, LBNL; however, it might be generalized for similar sample holder designs for both synchrotron and table-top microscopes. For complete details on the use and execution of this profile, please refer to Loconte et al. (2021).

SUBMITTER: Chen JH 

PROVIDER: S-EPMC8844304 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature