Investigating binding dynamics of trans resveratrol to HSA for an efficient displacement of aflatoxin B₁ using spectroscopy and molecular simulation.

ABSTRACT: Resveratrol is a polyphenol belonging to the class stilbenes. The active and stable form of resveratrol is trans-resveratrol. This polyphenol is bestowed with numerous biological properties. Aflatoxin B₁ is a hepato-carcinogen and mutagen that is produced by Aspergillus species. In this study, the interaction of trans-resveratrol with HSA followed by competitive dislodging of AFB₁ from HSA by trans-resveratrol has been investigated using spectroscopic studies. The UV-absorption studies revealed ground state complex formation between HSA and trans-resveratrol. Trans-resveratrol binds strongly to HSA with the binding constant of ~ 10⁷ M^-1 to a single binding site (n = 1.58), at 298.15 K. The Stern-Volmer quenching constant was calculated as 7.83 × 

SUBMITTER: Qureshi MA 

PROVIDER: S-EPMC8844415 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature