Super-Multiplex Nonlinear Optical Imaging Unscrambles the Statistical Complexity of Cancer Subtypes and Tumor Microenvironment.

ABSTRACT: Label-free nonlinear optical imaging (NLOI) has made tremendous inroads toward unscrambling the microcosmic complexity of cancers. However, harmonic and Raman microscopy offers throughput without redox information to reveal metabolic differentiation, and fluorescence lifetime microscopy lacks the vibrational response of molecules to visualize specific molecular constituents such as lipid. Here, a flexible, robust simultaneous multi-nonlinear imaging and cross-modality system that combines complementary imaging contrast mechanisms is demonstrated. This system, utilizing multiplexed ultrashort pulses, ingeniously integrates typical nonlinear processes, and high-dimension lifetime extension in a single setup to enhance the imaging dimensions and quality. Using this system, the authors perform

SUBMITTER: Li Y 

PROVIDER: S-EPMC8844469 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature