Angiogenin promotes angiogenesis via the endonucleolytic decay of miR-141 in colorectal cancer.

ABSTRACT: Mature microRNA (miRNA) decay is a key step in miRNA turnover and gene expression regulation. Angiogenin (ANG), the first human tumor-derived angiogenic protein and also a member of the RNase A superfamily, can promote tumor growth and metastasis by regulating rRNA biogenesis and tiRNA production. However, its effect on miRNA has not been explored. In this study, we find that ANG exclusively downregulates mature miR-141 in human umbilical endothelial cells (HUVECs) via its ribonuclease activity and preferably cleaves single-stranded miR-141 at the A⁵/C⁶, U⁷/G⁸, and U¹⁴/A¹⁵ sites via endonucleolytic digestion. By downregulating miR-141, ANG promotes HUVECs proliferation, migration, tube formation, and angiogenesis both in 

SUBMITTER: Weng C 

PROVIDER: S-EPMC8844805 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature