Generation of human induced pluripotent stem cell-derived cerebral organoids for cellular and molecular characterization.
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ABSTRACT:
SUBMITTER: Anastasaki C
PROVIDER: S-EPMC8844852 | biostudies-literature | 2022 Mar
REPOSITORIES: biostudies-literature
Publications
Generation of human induced pluripotent stem cell-derived cerebral organoids for cellular and molecular characterization.
Anastasaki Corina C Wilson Anna F AF Chen Alexander S AS Wegscheid Michelle L ML Gutmann David H DH
STAR protocols 20220208 1
Human induced pluripotent stem cell (hiPSC)-derived cerebral organoids (COs) can serve as an <i>in vitro</i> model for studying normal and pathologic human brain development. Here, we optimized existing protocols to streamline the generation of forebrain COs from hiPSCs. We employ these COs to define the impact of disease-causing mutations on cell fate, differentiation, maturation, and morphology relevant to neurodevelopmental disorders. Although limited to forebrain CO identity, this schema req ...[more]
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