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Protocol for glial Ca²⁺ imaging in C. elegans following chemical, mechanical, or optogenetic stimulation.

ABSTRACT: Caenorhabditis elegans is an exceptionally transparent model to analyze calcium (Ca²⁺) signals, but available protocols for neuronal Ca2⁺ imaging may not be suitable for studying glial cells. Here, we present a detailed protocol for glial Ca²⁺ imaging in C. elegans following three different approaches including chemical, mechanical, and optogenetic stimulation. We also provide the details for imaging analysis using Image-J. For complete details on the use and execution of this protocol, please refer to Duan et al. (2020).

SUBMITTER: Cheng H

PROVIDER: S-EPMC8844900 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Protocol for glial Ca2+ imaging in C. elegans following chemical, mechanical, or optogenetic stimulation.

Cheng Hankui H Al-Sheikh Umar U Chen Du D Duan Duo D Kang Lijun L

STAR protocols 20220210 1

Caenorhabditis elegans is an exceptionally transparent model to analyze calcium (Ca2+) signals, but available protocols for neuronal Ca2+ imaging may not be suitable for studying glial cells. Here, we present a detailed protocol for glial Ca2+ imaging in C. elegans following three different approaches including chemical, mechanical, and optogenetic stimulation. We also provide the details for imaging analysis using Image-J. For complete details on t ...[more]

PMID: 35199034

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