Inhibition of Protein <i>N-</i>Glycosylation Blocks SARS-CoV-2 Infection.

ABSTRACT: Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) extensively N-glycosylates its spike proteins, which are necessary for host cell invasion and the target of both vaccines and immunotherapies. These N-glycans are predicted to modulate spike binding to the host receptor by stabilizing its open conformation and host immunity evasion. Here, we investigated the essentiality of both the host N-glycosylation pathway and SARS-CoV-2 N-glycans for infection. Ablation of host N-glycosylation using RNA interference or inhibitors, including FDA-approved drugs, reduced the spread of the infection, including that of variants B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma) and B.1.617.2 (Delta). Under these conditions, cells produced fewer virions and some compl

SUBMITTER: Casas-Sanchez A 

PROVIDER: S-EPMC8844921 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature