An efficient and specific CRISPR-Cas9 genome editing system targeting soybean phytoene desaturase genes.

ABSTRACT:

Background

Genome editing by CRISPR/Cas9 has become a popular approach to induce targeted mutations for crop trait improvement. Soybean (Glycine max L. Merr.) is an economically important crop worldwide. Although gene editing has been demonstrated in soybean, its utilization in stably transformed plants through whole plant regeneration is still not widespread, largely due to difficulties with transformation or low mutation efficiencies.

Results

We sought to establish a simple, efficient, and specific CRISPR/Cas9 system to induce heritable mutations in soybean through stable transformation. We targeted phytoene desaturase (PDS) genes due to the distinctive dwarf and albino phenotypes of the loss of function mutant. To evaluate gene editing efficiency and specificity, three co

SUBMITTER: Lu QSM 

PROVIDER: S-EPMC8845245 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature