Label-Free Raman Microspectroscopy for Identifying Prokaryotic Virocells.

ABSTRACT: Raman microspectroscopy has been used to thoroughly assess growth dynamics and heterogeneity of prokaryotic cells, yet little is known about how the chemistry of individual cells changes during infection with virulent viruses, resulting in so-called virocells. Here, we investigate biochemical changes of bacterial and archaeal cells of three different species in laboratory cultures before and after addition of their respective viruses using single-cell Raman microspectroscopy. By applying multivariate statistics, we identified significant differences in the spectra of single cells with/without addition of virulent dsRNA phage (phi6) for Pseudomonas syringae. A general ratio of wavenumbers that contributed the greatest differences in the recorded spectra was defined as an indicator fo

SUBMITTER: Monsees I 

PROVIDER: S-EPMC8845568 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature