Optimization of an economical medium composition for the coculture of Clostridium butyricum and Bacillus coagulans.

ABSTRACT: Clostridium butyricum is a spore-forming probiotic which can promote the enhancement of beneficial bacteria and maintain intestinal microecological balance. However, it is difficult to improve the production level of C. butyricum by conventional fermentation process. In this study, a co-fermentation process of C. butyricum DL-1 and Bacillus coagulans ZC2-1 was established to improve the viable counts and spore yield of C. butyricum, and the formula of coculture medium was optimized by flask fermentation. The results showed that the optimum medium composition is 10 g/L bran, 15 g/L corn steep powder, 15 g/L peptone, 1 g/L K₂HPO₄ and 0.5 g/L MnSO₄.Cultured stationarily in the optimal medium for 36 h, the number of viable bacteria of C. butyricum DL-1 reached

SUBMITTER: Li Y 

PROVIDER: S-EPMC8847521 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature