An improved ovine reference genome assembly to facilitate in-depth functional annotation of the sheep genome.

ABSTRACT:

Background

The domestic sheep (Ovis aries) is an important agricultural species raised for meat, wool, and milk across the world. A high-quality reference genome for this species enhances the ability to discover genetic mechanisms influencing biological traits. Furthermore, a high-quality reference genome allows for precise functional annotation of gene regulatory elements. The rapid advances in genome assembly algorithms and emergence of sequencing technologies with increasingly long reads provide the opportunity for an improved de novo assembly of the sheep reference genome.

Findings

Short-read Illumina (55× coverage), long-read Pacific Biosciences (75× coverage), and Hi-C data from this ewe retrieved from public databases were combined with an additional 50× coverage of O

SUBMITTER: Davenport KM 

PROVIDER: S-EPMC8848310 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature