Qiber3D-an open-source software package for the quantitative analysis of networks from 3D image stacks.

ABSTRACT:

Background

Optical slice microscopy is commonly used to observe cellular morphology in 3D tissue culture, e.g., the formation of cell-derived networks. The morphometric quantification of these networks is essential to study the cellular phenotype. Commonly, the quantitative measurements are performed on 2D projections of the image stack, resulting in the loss of information in the third dimension. Currently available 3D image analysis tools rely on manual interactions with the software and are therefore not feasible for large datasets.

Findings

Here we present Qiber3D, an open-source image processing toolkit. The software package includes the essential image analysis procedures required for image processing, from the raw image to the quantified data. Optional pre-processing

SUBMITTER: Jaeschke A 

PROVIDER: S-EPMC8848317 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature