A novel combined fluorescent probe staining method for circulating tumor cell identification.

ABSTRACT:

Background

To develop a novel highly accurate circulating tumor cell (CTC) identification method and to validate its application in cancer diagnostics and/or prognostics.

Methods

We verified and validated the combined fluorescent probe staining protocol (combination of three fluorescent probes: Dil, Hoechst 33342, and PY) through CTC and non-CTC (white blood cell) morphological comparison of five tumor cell lines (THP-1, HEC, HEPG2, Eca-109, HeLa) in vitro and 32 patient tumor samples from the Shandong Cancer Hospital and Institute. Wright's Giemsa staining and cluster differentiation 45 (CD45) immunocytochemistry (ICC) staining were used as reference control methods. The association between the developed method and clinicopathology was also investigated.

Results</

SUBMITTER: Wang H 

PROVIDER: S-EPMC8848375 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature