Normothermic ex vivo lung perfusion outperforms conventional cold preservation in a deceased rat lung.

ABSTRACT:

Background

Utilization rate of donor lungs in China is constrained by the inefficiency of conventional cold preservation (COLD) in lung transplantation. We assessed the performance of normothermic ex vivo lung perfusion (EVLP) in lung donors following cardiac death (DCD) with fresh EVLP perfusate.

Methods

A total of 24 adult Sprague-Dawley (SD) rats were randomized into three groups (EVLP, COLD, and control groups) with 8 rats each. Fresh EVLP perfusate was prepared for lung perfusion. The pH, electrolyte concentration, perfusate penetration pressure during irrigation, physiological function, integrity of barrier function, pathological changes, and expressions of inflammation-related cytokines of donor lungs were examined.

Results

The pH, electrolyte concentration, os

SUBMITTER: Wang W 

PROVIDER: S-EPMC8848380 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature