Influence of bFGF on in vitro expansion and chondrogenic construction of articular cartilage-derived progenitor cells.

ABSTRACT:

Background

Articular cartilage-derived progenitor cells (ACPCs) possess the properties of both chondrocytes and bone marrow mesenchymal stem cells (BMSCs). However, the number of ACPCs in articular cartilage is low, and an effective culture system is needed for their expansion. Basic fibroblast growth factor (bFGF) promotes the expansion of chondrocytes and BMSCs, as well as the chondrogenic differentiation of BMSCs. Therefore, the aim of this study was to clarify whether bFGF could be used for in vitro expansion of ACPCs and whether bFGF promoted chondrogenic construction of ACPCs.

Methods

We applied the fibronectin adhesion method to sort mice ACPCs and compared the proliferative, osteogenic, and chondrogenic abilities of ACPCs by adding various concentration of bFG

SUBMITTER: Shen X 

PROVIDER: S-EPMC8848382 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature