4-Dimensional Imaging of Zebrafish Optic Cup Morphogenesis.

ABSTRACT: Visual system function requires the establishment of precise tissue and organ structures. In the vertebrate eye, structural defects are a common cause of visual impairment, yet mechanisms of eye morphogenesis are still poorly understood. The basic organization of the embryonic eye is conserved throughout vertebrates, thus live imaging of zebrafish embryos has become a powerful approach to directly observe eye development at real time under normal and pathological conditions. Dynamic cell processes including movements, morphologies, interactions, division, and death can be visualized in the embryo. We have developed methods for uniform labeling of subcellular structures and timelapse confocal microscopy of early eye development in zebrafish. This protocol outlines the method of generating c

SUBMITTER: Lusk S 

PROVIDER: S-EPMC8848516 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature