Overexpression of CBS/H₂S inhibits proliferation and metastasis of colon cancer cells through downregulation of CD44.

ABSTRACT:

Background

The role of hydrogen sulfide (H₂S) in cancer biology is controversial, including colorectal cancer. The bell-shaped effect of H₂S refers to pro-cancer action at lower doses and anti-cancer effect at higher concentrations. We hypothesized that overexpression of cystathionine-beta-synthase (CBS)/H₂S exerts an inhibitory effect on colon cancer cell proliferation and metastasis.

Methods

Cell proliferation was assessed by Cell Counting Kit-8 (CCK-8), clone-formation and sphere formation assay. Cell migration was evaluated by transwell migration assay. Intracellular H₂S was detected by H₂S probe. Chromatin immunoprecipitation (ChIP) analysis was carried out to examine DNA-protein interaction. Cell experiments also i

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC8848668 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature