Increased oxidative stress contributes to impaired peripheral CD56^dimCD57⁺ NK cells from patients with systemic lupus erythematosus.

ABSTRACT:

Background

Systemic lupus erythematosus (SLE) is characterized by loss of immune tolerance and imbalance of immune cell subsets. Natural killer (NK) cells contribute to regulate both the innate and adaptive immune response. In this study, we aimed to detect alterations of peripheral NK cells and explore intrinsic mechanisms involving in NK cell abnormality in SLE.

Methods

Blood samples from healthy controls (HCs) and patients with SLE and rheumatoid arthritis (RA) were collected. The NK count, NK subsets (CD56^bright, CD56^dimCD57^-, and CD56^dimCD57⁺), phenotypes, and apoptosis were evaluated with flow cytometer. Mitochondrial reactive oxygen species (mtROS) and total ROS levels were detected with MitoSOX Red and DCFH-DA st

SUBMITTER: Lu Z 

PROVIDER: S-EPMC8848960 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature