Tollip interaction with STAT3: a novel mechanism to regulate human airway epithelial responses to type 2 cytokines.

ABSTRACT:

Background

Toll-interacting protein (Tollip) is one of the key negative regulators in host innate immunity. Genetic variation of Tollip has been associated with less Tollip expression and poor lung function in asthmatic patients, but little is known about the role of Tollip in human airway type 2 inflammatory response, a prominent feature in allergic asthma.

Objective

Our goal was to determine the role and underlying mechanisms of Tollip in human airway epithelial responses such as eotaxin to type 2 cytokine IL-13.

Methods

Tollip deficient primary human airway epithelial cells from 4 healthy donors were generated by the gene knockdown approach and stimulated with IL-13 to measure activation of transcription factor STAT3, and eotaxin-3, an eosinophilic chemokine.

Re

SUBMITTER: Schaunaman N 

PROVIDER: S-EPMC8848971 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature