Intravital deep-tumor single-beam 3-photon, 4-photon, and harmonic microscopy.

ABSTRACT: Three-photon excitation has recently been demonstrated as an effective method to perform intravital microscopy in deep, previously inaccessible regions of the mouse brain. The applicability of 3-photon excitation for deep imaging of other, more heterogeneous tissue types has been much less explored. In this work, we analyze the benefit of high-pulse-energy 1 MHz pulse-repetition-rate infrared excitation near 1300 and 1700 nm for in-depth imaging of tumorous and bone tissue. We show that this excitation regime provides a more than 2-fold increased imaging depth in tumor and bone tissue compared to the illumination conditions commonly used in 2-photon excitation, due to improved excitation confinement and reduced scattering. We also show that simultaneous 3- and 4-photon processes can be eff

SUBMITTER: Bakker GJ 

PROVIDER: S-EPMC8849342 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature