The SAMBA tool uses long reads to improve the contiguity of genome assemblies.

ABSTRACT: Third-generation sequencing technologies can generate very long reads with relatively high error rates. The lengths of the reads, which sometimes exceed one million bases, make them invaluable for resolving complex repeats that cannot be assembled using shorter reads. Many high-quality genome assemblies have already been produced, curated, and annotated using the previous generation of sequencing data, and full re-assembly of these genomes with long reads is not always practical or cost-effective. One strategy to upgrade existing assemblies is to generate additional coverage using long-read data, and add that to the previously assembled contigs. SAMBA is a tool that is designed to scaffold and gap-fill existing genome assemblies with additional long-read data, resulting in substantially gr

SUBMITTER: Zimin AV 

PROVIDER: S-EPMC8849508 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature