Estimating the glutamate transporter surface density in distinct sub-cellular compartments of mouse hippocampal astrocytes.

ABSTRACT: Glutamate transporters preserve the spatial specificity of synaptic transmission by limiting glutamate diffusion away from the synaptic cleft, and prevent excitotoxicity by keeping the extracellular concentration of glutamate at low nanomolar levels. Glutamate transporters are abundantly expressed in astrocytes, and previous estimates have been obtained about their surface expression in astrocytes of the rat hippocampus and cerebellum. Analogous estimates for the mouse hippocampus are currently not available. In this work, we derive the surface density of astrocytic glutamate transporters in mice of different ages via quantitative dot blot. We find that the surface density of glial glutamate transporters is similar in 7-8 week old mice and rats. In mice, the levels of glutamate transporter

SUBMITTER: Radulescu AR 

PROVIDER: S-EPMC8849624 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature