Effective bubble-based testing for SARS-CoV-2 using swab-pooling.

ABSTRACT:

Objectives

Despite the success in developing COVID-19 vaccines, containment of the disease is obstructed worldwide by vaccine production bottlenecks, logistics hurdles, vaccine refusal, transmission through unvaccinated children, and the appearance of new viral variants. This underscores the need for effective strategies for identifying carriers/patients, which was the main aim of this study.

Methods

We present a bubble-based PCR testing approach using swab-pooling into lysis buffer. A bubble is a cluster of people who can be periodically tested for SARS-CoV-2 by swab-pooling. A positive test of a pool mandates quarantining each of its members, who are then individually tested while in isolation to identify the carrier(s) for further epidemiological contact tracing.

Resul

SUBMITTER: Cohen Y 

PROVIDER: S-EPMC8849906 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature