An efficient urine peptidomics workflow identifies chemically defined dietary gluten peptides from patients with celiac disease.

ABSTRACT: Celiac disease (CeD) is an autoimmune disorder induced by consuming gluten proteins from wheat, barley, and rye. Glutens resist gastrointestinal proteolysis, resulting in peptides that elicit inflammation in patients with CeD. Despite well-established connections between glutens and CeD, chemically defined, bioavailable peptides produced from dietary proteins have never been identified from humans in an unbiased manner. This is largely attributable to technical challenges, impeding our knowledge of potentially diverse peptide species that encounter the immune system. Here, we develop a liquid chromatographic-mass spectrometric workflow for untargeted sequence analysis of the urinary peptidome. We detect over 600 distinct dietary peptides, of which ~35% have a CeD-relevant T cell epitope an

SUBMITTER: Palanski BA 

PROVIDER: S-EPMC8850430 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature