Site-Specific Sequence Exchange Between Homologous and Non-homologous Chromosomes.

ABSTRACT: Transgene integration typically takes place in an easy-to-transform laboratory variety before the transformation event is introgressed through backcrosses to elite cultivars. As new traits are added to existing transgenic lines, site-specific integration can stack new transgenes into a previously created transgenic locus. In planta site-specific integration minimizes the number of segregating loci to assemble into a breeding line, but cannot break genetic linkage between the transgenic locus and nearby undesirable traits. In this study, we describe an additional feature of an in planta gene-stacking scheme, in which the Cre (control of recombination) recombinase not only deletes transgenic DNA no longer needed after transformation but also mediates recombination between homol

SUBMITTER: Yin Q 

PROVIDER: S-EPMC8850970 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature