ATP Synthase K⁺- and H⁺-fluxes Drive ATP Synthesis and Enable Mitochondrial K⁺-"Uniporter" Function: II. Ion and ATP Synthase Flux Regulation.

ABSTRACT: We demonstrated that ATP synthase serves the functions of a primary mitochondrial K⁺ "uniporter," i.e., the primary way for K⁺ to enter mitochondria. This K⁺ entry is proportional to ATP synthesis, regulating matrix volume and energy supply-vs-demand matching. We show that ATP synthase can be upregulated by endogenous survival-related proteins via IF₁. We identified a conserved BH3-like domain of IF₁ which overlaps its "minimal inhibitory domain" that binds to the β-subunit of F₁. Bcl-xL and Mcl-1 possess a BH3-binding-groove that can engage IF₁ and exert effects, requiring this interaction, comparable to diazoxide to augment ATP synthase's H⁺ and K⁺ flux and ATP synthesis. Bcl-xL and

SUBMITTER: Juhaszova M 

PROVIDER: S-EPMC8850977 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature