Nonmuscle Myosin II is Required for Larval Shell Formation in a Patellogastropod.

ABSTRACT: The molecular mechanisms underlying larval shell development in mollusks remain largely elusive. We previously found evident filamentous actin (F-actin) aggregations in the developing shell field of the patellogastropod Lottia goshimai, indicating roles of actomyosin networks in the process. In the present study, we functionally characterized nonmuscle myosin II (NM II), the key molecule in actomyosin networks, in the larval shell development of L. goshimai. Immunostaining revealed general colocalization of phosphorylated NM II and F-actin in the shell field. When inhibiting the phosphorylation of NM II using the specific inhibitor blebbistatin in one- or 2-h periods during shell field morphogenesis (6-8 h post-fertilization, hpf), the larval shell plate was completely lost i

SUBMITTER: Liu X 

PROVIDER: S-EPMC8851382 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature