Modular 3D-Printed Peg Biofilm Device for Flexible Setup of Surface-Related Biofilm Studies.

ABSTRACT: Medical device-related biofilms are a major cause of hospital-acquired infections, especially chronic infections. Numerous diverse models to study surface-associated biofilms have been developed; however, their usability varies. Often, a simple method is desired without sacrificing throughput and biological relevance. Here, we present an in-house developed 3D-printed device (FlexiPeg) for biofilm growth, conceptually similar to the Calgary Biofilm device but aimed at increasing ease of use and versatility. Our device is modular with the lid and pegs as separate units, enabling flexible assembly with up- or down-scaling depending on the aims of the study. It also allows easy handling of individual pegs, especially when disruption of biofilm populations is needed for downstream analysis. The

SUBMITTER: Zaborskyte G 

PROVIDER: S-EPMC8851424 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature