A New Rapid and Quantitative Assay to Determine the Phytase Activity of Feed.

ABSTRACT: The fortification of animal feed with enzymes in order to optimize feed utilization has become a standard for the meat production industry. A method for measuring levels of active enzymes that can be carried out quickly would ensure that feed has been supplemented with the appropriate amount of enzyme. Phytase is the most widely used feed enzyme and is routinely quantified with an activity assay in a limited number of specialized laboratories. As an alternative, we report here the development of a rapid and easy method to perform a quantitative assay for the phytase from Citrobacter braakii. The method is suitable for use at local sites with a minimum lab setup and will reduce delays and potential interferences due to improper sample storage and shipment. The new assay is based on a

SUBMITTER: Willard ECD 

PROVIDER: S-EPMC8851631 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature