Ontology highlight
ABSTRACT:
SUBMITTER: Zhang X
PROVIDER: S-EPMC8852347 | biostudies-literature | 2022 Feb
REPOSITORIES: biostudies-literature
Zhang Xi X Zhang Biao B Freddolino Lydia L Zhang Yang Y
Nature methods 20220207 2
Cryo-electron microscopy (cryo-EM) has become a leading approach for protein structure determination, but it remains challenging to accurately model atomic structures with cryo-EM density maps. We propose a hybrid method, CR-I-TASSER (cryo-EM iterative threading assembly refinement), which integrates deep neural-network learning with I-TASSER assembly simulations for automated cryo-EM structure determination. The method is benchmarked on 778 proteins with simulated and experimental density maps, ...[more]