ChREBP-regulated lipogenesis is not required for the thermogenesis of brown adipose tissue.

ABSTRACT:

Objectives

Brown adipose tissue (BAT) plays a critical role in energy expenditure by uncoupling protein 1 (UCP1)-mediated thermogenesis and represents an important therapeutic target for metabolic diseases. Carbohydrate response element-binding protein (ChREBP) is a key transcription factor regulating de novo lipogenesis, and its activity is associated with UCP1 expression and thermogenesis in BAT. However, the exact physiological role of endogenous ChREBP in BAT thermogenesis remains unclear.

Methods

We used the Cre/LoxP system to generate ChREBP BAT-specific knockout mice, and examined their BAT thermogenesis under acute cold exposure and long-term cold acclimation. Gene expression was analyzed at the mRNA and protein levels, and lipogenesis was examined by ³H-H

SUBMITTER: Wei C 

PROVIDER: S-EPMC8853070 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature