Protein visualization and manipulation in Drosophila through the use of epitope tags recognized by nanobodies.

ABSTRACT: Expansion of the available repertoire of reagents for visualization and manipulation of proteins will help understand their function. Short epitope tags linked to proteins of interest and recognized by existing binders such as nanobodies facilitate protein studies by obviating the need to isolate new antibodies directed against them. Nanobodies have several advantages over conventional antibodies, as they can be expressed and used as tools for visualization and manipulation of proteins in vivo. Here, we characterize two short (<15aa) NanoTag epitopes, 127D01 and VHH05, and their corresponding high-affinity nanobodies. We demonstrate their use in Drosophila for in vivo protein detection and re-localization, direct and indirect immunofluorescence, immunoblotting, and immunoprecipitati

SUBMITTER: Xu J 

PROVIDER: S-EPMC8853664 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature