Dataset Information

¹H, ¹³C and ¹⁵N chemical shift assignment of the stem-loops 5b + c from the 5'-UTR of SARS-CoV-2.

ABSTRACT: The ongoing pandemic of the respiratory disease COVID-19 is caused by the SARS-CoV-2 (SCoV2) virus. SCoV2 is a member of the Betacoronavirus genus. The 30 kb positive sense, single stranded RNA genome of SCoV2 features 5'- and 3'-genomic ends that are highly conserved among Betacoronaviruses. These genomic ends contain structured cis-acting RNA elements, which are involved in the regulation of viral replication and translation. Structural information about these potential antiviral drug targets supports the development of novel classes of therapeutics against COVID-19. The highly conserved branched stem-loop 5 (SL5) found within the 5'-untranslated region (5'-UTR) consists of a basal stem and three stem-loops, namely SL5a, SL5b and SL5c. Both, SL5a and SL5b feature a 5'-UUUCGU-3' hexaloop that is also found among Alphacoronaviruses. Here, we report the extensive ¹H, ¹³C and ¹⁵N resonance assignment of the 37 nucleotides (nts) long sequence spanning SL5b and SL5c (SL5b + c), as basis for further in-depth structural studies by solution NMR spectroscopy.

SUBMITTER: Mertinkus KR

PROVIDER: S-EPMC8853908 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature

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1H, 13C and 15N chemical shift assignment of the stem-loops 5b + c from the 5'-UTR of SARS-CoV-2.

Mertinkus Klara R KR Grün J Tassilo JT Altincekic Nadide N Bains Jasleen Kaur JK Ceylan Betül B Ferner Jan-Peter JP Frydman Lucio L Fürtig Boris B Hengesbach Martin M Hohmann Katharina F KF Hymon Daniel D Kim Jihyun J Knezic Božana B Novakovic Mihajlo M Oxenfarth Andreas A Peter Stephen A SA Qureshi Nusrat S NS Richter Christian C Scherf Tali T Schlundt Andreas A Schnieders Robbin R Schwalbe Harald H Stirnal Elke E Sudakov Alexey A Vögele Jennifer J Wacker Anna A Weigand Julia E JE Wirmer-Bartoschek Julia J Martin Maria A Wirtz MAW Wöhnert Jens J

Biomolecular NMR assignments 20220218 1

The ongoing pandemic of the respiratory disease COVID-19 is caused by the SARS-CoV-2 (SCoV2) virus. SCoV2 is a member of the Betacoronavirus genus. The 30 kb positive sense, single stranded RNA genome of SCoV2 features 5'- and 3'-genomic ends that are highly conserved among Betacoronaviruses. These genomic ends contain structured cis-acting RNA elements, which are involved in the regulation of viral replication and translation. Structural information about these potential antiviral drug targets ...[more]

PMID: 35178672

Dataset Information

¹H, ¹³C and ¹⁵N chemical shift assignment of the stem-loops 5b + c from the 5'-UTR of SARS-CoV-2.

Publications

<sup>1</sup>H, <sup>13</sup>C and <sup>15</sup>N chemical shift assignment of the stem-loops 5b + c from the 5'-UTR of SARS-CoV-2.

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