Mitochondrial homeostasis regulates definitive endoderm differentiation of human pluripotent stem cells.

ABSTRACT: Cellular organelles play fundamental roles in almost all cell behaviors. Mitochondria have been reported to be functionally linked to various biological processes, including reprogramming and pluripotency maintenance. However, very little about the role of mitochondria has been revealed in human early development and lineage specification. Here, we reported the characteristics and function of mitochondria during human definitive endoderm differentiation. Using a well-established differentiation system, we first investigated the change of mitochondrial morphology by comparing undifferentiated pluripotent stem cells, the intermediate mesendoderm cells, and differentiated endoderm cells, and found that mitochondria were gradually elongated and matured along differentiation. We further analyze

SUBMITTER: Lv J 

PROVIDER: S-EPMC8854419 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature