Optimized NMR Experiments for the Isolation of I=1/2 Manifold Transitions in Methyl Groups of Proteins.

ABSTRACT: Optimized NMR experiments are developed for isolating magnetization belonging to the I=1/2 manifolds of ¹³ CH₃ methyl groups in proteins, enabling the manipulation of the magnetization of a ¹³ CH₃ moiety as if it were an AX (¹ H-¹³ C) spin-system. These experiments result in the same 'simplification' of a ¹³ CH₃ spin-system that would be obtained from the production of {¹³ CHD₂ }-methyl-labeled protein samples. The sensitivity of I=1/2 manifold-selection experiments is a factor of approximately 2 less than that of the corresponding experiments acquired on {¹³ CHD₂ }-labeled methyl groups. The methodology described here is primarily intended for small-to-medium

SUBMITTER: Tugarinov V 

PROVIDER: S-EPMC8855706 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature