Comparing DNA yield from fish scales following different extraction protocols.

ABSTRACT: Studies on genetic diversity, adaptive potential and fitness of species have become a major tool in conservation biology. These studies require biological material containing a reliable source of DNA which can be extracted and analysed. Recently, non-invasive sampling has become the preferred sampling method of such biological material; particularly when studying endangered species. Elasmoid scales from teleost fish are an example of non-invasive samples from which DNA can successfully be extracted. This study compared different extraction protocols to find an optimal method for extracting DNA from teleost fish scales. This was done with the intent to use the protocol that yielded the highest quantity of DNA on dried, archived scales. The protocols tested in this study included (1) phenol/

SUBMITTER: Shuttleworth L 

PROVIDER: S-EPMC8857249 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature