Efficient genome editing in Claviceps purpurea using a CRISPR/Cas9 ribonucleoprotein method.

ABSTRACT: Claviceps purpurea produces many pharmacologically important ergot alkaloids (EAS), which are widely used to treat migraine and hypertension and to aid childbirth. Although an EAS biosynthetic cluster of C. purpurea has been discovered more than 20 years ago, the complete biosynthetic pathway of EAS has not been fully characterized until now. The main obstacle to elucidating this pathway and strain modification is the lack of efficient genome-editing tools for C. purpurea. The conventional gene manipulation method for C. purpurea relies on homologous recombination (HR), although the efficiency of HR in C. purpurea is very low (∼1-5%). Consequently, the disruption of target genes is laborious and time-consuming. Although CRISPR/

SUBMITTER: Yu L 

PROVIDER: S-EPMC8857428 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature