A FAIR-compliant parts catalogue for genome engineering and expression control in Saccharomyces cerevisiae.

ABSTRACT: The synthetic biology toolkit for baker's yeast, Saccharomyces cerevisiae, includes extensive genome engineering toolkits and parts repositories. However, with the increasing complexity of engineering tasks and versatile applications of this model eukaryote, there is a continued interest to expand and diversify the rational engineering capabilities in this chassis by FAIR (findable, accessible, interoperable, and reproducible) compliance. In this study, we designed and characterised 41 synthetic guide RNA sequences to expand the CRISPR-based genome engineering capabilities for easy and efficient replacement of genomically encoded elements. Moreover, we characterize in high temporal resolution 20 native promoters and 18 terminators using fluorescein and LUDOX CL-X as references for G

SUBMITTER: D'Ambrosio V 

PROVIDER: S-EPMC8857431 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature