The METTL5-TRMT112 N⁶-methyladenosine methyltransferase complex regulates mRNA translation via 18S rRNA methylation.

ABSTRACT: Ribosomal RNAs (rRNAs) have long been known to carry chemical modifications, including 2'O-methylation, pseudouridylation, N⁶-methyladenosine (m⁶A), and N^6,6-dimethyladenosine. While the functions of many of these modifications are unclear, some are highly conserved and occur in regions of the ribosome critical for mRNA decoding. Both 28S rRNA and 18S rRNA carry single m⁶A sites, and while the methyltransferase ZCCHC4 has been identified as the enzyme responsible for the 28S rRNA m⁶A modification, the methyltransferase responsible for the 18S rRNA m⁶A modification has remained unclear. Here, we show that the METTL5-TRMT112 methyltransferase complex installs the m⁶A modification at position 1832 of human 18S rRNA.

SUBMITTER: Sepich-Poore C 

PROVIDER: S-EPMC8857481 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature