Capturing membrane trafficking events during 3D angiogenic development in vitro.

ABSTRACT:

Objectives

Vesicular trafficking dictates protein localization, functional activity, and half-life, providing a critically important regulatory step in tissue development; however, there is little information detailing endothelial-specific trafficking signatures. This is due, in part, to limitations in visualizing trafficking events in endothelial tissues. Our aim in this investigation was to explore the use of a 3-dimensional (3D) in vitro sprouting model to image endothelial membrane trafficking events.

Methods

Endothelial cells were challenged to grow sprouts in a fibrin bead assay. Thereafter, spouts were transfected with fluorescent proteins and stained for various cell markers. Sprouts were then imaged for trafficking events using live and fixed-cell microscopy.

Res

SUBMITTER: Francis CR 

PROVIDER: S-EPMC8858330 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature