Litmus-Body: A Molecularly Targeted Sensor for Cell-Surface pH Measurements.

ABSTRACT: Precise pH measurements in the immediate environment of receptors is essential for elucidating the mechanisms through which local pH changes associated with diseased phenotypes manifest into aberrant receptor function. However, current pH sensors lack the ability to localize and target specific receptor molecules required to make these measurements. Herein we present the Litmus-body, our recombinant protein-based pH sensor, which through fusion to an anti-IgG nanobody is capable of piggybacking on IgG antibodies for molecular targeting to specific proteins on the cell surface. By normalizing a pH-dependent green fluorescent protein to a long Stokes shift red fluorophore or fluorescent protein, we readily report pH independent of sensor concentration using a single 488 nm excitation. Our Li

SUBMITTER: Kuo JC 

PROVIDER: S-EPMC8858598 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature