The Influence of Temperature on the Antiviral Response of mIgM⁺ B Lymphocytes Against Hirame Novirhabdovirus in Flounder (Paralichthys olivaceus).

ABSTRACT: Hirame novirhabdovirus (HIRRV) is an ongoing threat to the aquaculture industry. The water temperature for the onset of HIRRV is below 15°C, the peak is about 10°C, but no mortality is observed over 20°C. Previous studies found the positive signal of matrix protein of HIRRV (HIRRV-M) was detected in the peripheral blood leukocytes of viral-infected flounder. Flow cytometry and indirect immunofluorescence assay showed that HIRRV-M was detected in mIgM⁺ B lymphocytes in viral-infected flounder maintained at 10°C and 20°C, and 22% mIgM⁺ B lymphocytes are infected at 10°C while 13% are infected at 20°C, indicating that HIRRV could invade into mIgM⁺ B lymphocytes. Absolute quantitative RT-PCR showed that the viral copies in mIgM⁺ B lymphocytes

SUBMITTER: Tang X 

PROVIDER: S-EPMC8858815 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature