Host-Dependent Producibility of Recombinant Cypridina noctiluca Luciferase With Glycosylation Defects.

ABSTRACT: Cypridina noctiluca luciferase (CLuc) is a secreted luminescent protein that reacts with its substrate (Cypridina luciferin) to emit light. CLuc is known to be a thermostable protein and has been used for various research applications, including in vivo imaging and high-throughput reporter assays. Previously, we produced a large amount of recombinant CLuc for crystallographic analysis. However, this recombinant protein did not crystallize, probably due to heterogeneous N-glycan modifications. In this study, we produced recombinant CLuc without glycan modifications by introducing mutations at the N-glycan modification residues using mammalian Expi293F cells, silkworms, and tobacco Bright Yellow-2 cells. Interestingly, recombinant CLuc production depended heavily on the express

SUBMITTER: Mitani Y 

PROVIDER: S-EPMC8859458 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature