Novel insights into the electrophysiology of murine cardiac macrophages: relevance of voltage-gated potassium channels.

ABSTRACT:

Aims

Macrophages (MΦ), known for immunological roles, such as phagocytosis and antigen presentation, have been found to electrotonically couple to cardiomyocytes (CM) of the atrioventricular node via Cx43, affecting cardiac conduction in isolated mouse hearts. Here, we characterize passive and active electrophysiological properties of murine cardiac resident MΦ, and model their potential electrophysiological relevance for CM.

Methods and results

We combined classic electrophysiological approaches with 3D florescence imaging, RNA-sequencing, pharmacological interventions, and computer simulations. We used Cx3cr1eYFP/+ mice wherein cardiac MΦ are fluorescently labelled. FACS-purified fluorescent MΦ from mouse hearts were studied by whole-cell patch-clamp. MΦ electrophysiologic

SUBMITTER: Simon-Chica A 

PROVIDER: S-EPMC8859634 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature